ABSTRACT
Microspheres (MS) are an excellent transarterial chemoembolization carrier for cancer treatment. Then the Bletilla striata polysaccharide (BSP) that was isolated from the rattan of Bletilla striata was used as skeleton material, and the matrine (ME) loaded Bletilla striata polysaccharide microspheres (ME-BSPMS) were prepared by emulsify-chemical crosslinking method. ME-BSPMS was characterized for appearance shape, particle size, drug loading, swelling ratio, suspension property, drug entrapment condition and in vitro release characteristics. The results showed that the ME-BSPMS appeared as round spherical and smooth shape by SEM, with an average size of (85 ±7) μm. ME-BSPMS with a good suspension in physiological saline and the swelling ratio could reach upwards of (53 ±4.2)% in 20 minutes, also with a large amount of drug loading of (30.12 ±3.25)%. The results of DSC scanning indicate that good compatibility exists between the ME and BSP, and the ME could be embedded fully in the matrix of the ME-BSPMS. The accumulation drug release from ME-BSPMS was (25.38 ±1.57)% at 12 h, this suggests that the ME-BSPMS has a good sustained release effect. These results indicate that the ME-BSPMS may be a promising transarterial chemoembolization carrier for cancer treatment.
ABSTRACT
Objective To identify the factors of no-reflow during emergency percutaneous coronary intervention (PCI) in patients with acute ST-segment elevation myocardial infarction (STEMI). Methods Atotal of 92 patients with acute STEMI treated with PCI were divided intonormal reflow group(73 patients) and no-reflow group (19 patients) from June 2012 to January 2013. The influencing factors of no-reflow were clarified by comparing clinical symptoms of the two groups. Results The no-reflow rate after undergoing emergency PCI was 20. 7 % in 92 acute STEMI patients. The systolic blood pressure (SBP), type 2 diabetes, troponin T peak, period from disease onset to reperfusion, balloon expansion times and stent number of the target blood vessel were significantly different between the two groups (P<0. 05). Multivariate logistic regression analysis identified that low SBP (SBP<100 mmHg, 1 mmHg=0. 133 kPa), type 2 diabetes, balloon expansion times, troponin T peak, right coronary artery lesions and period from disease onset to reperfusion were the risk factors for noreflow during PCI in patients with STEMI. Conclusion No-reflow following emergency PCI in acute STEMI patients is associatedwith six clinical factors: SBP<100 mmHg, type 2 diabetes, balloon expansion times, troponin T peak, right coronary artery lesions and period from disease onset to reperfusion.
ABSTRACT
Objective: To explore the effect of oxidized low-density lipoprotein (ox-LDL) on Notch 1 expression and cytokine secretion in human acute monocytic leukemia cell line THP1, so as to discuss the role of ox-LDL in atherosclerosis (AS). Methods: THP1 cells were stimulated with PMA to induce human macrophages, which were then exposed to ox-LDL of different concentrations. The morphological changes of the cells was observed by phase contrast microscope. The expression of Notch1 mRNA was examined by real-time quantitative PCR (RT-PCR) and protein expression was measured by Western blotting analysis in the cells. The levels of vascular cell adhesive molecule-1(VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in supernatants were determined by ELISA. Results: The macrophages differentiated into dendritic like cells 48 h after ox-LDL treatment. Ox-LDL treatment increased Notch1 mRNA and protein expression compared with the control group. Ox-LDL also significantly increased the levels of VCAM-1 and MCP-1 in the supernatant in a dose-dependent manner (P <0.05). The best effect of ox-LDL was seen at the concentration of 50 mg/L. Conclusion: Ox-LDL stimulation can increase Notch 1 expression in THP1 cells; meanwhile, it can also promote the secretion of AS-related cytokines. The role of ox-LDL in AS might be partly mediated by Notch1.